Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. The QC software provides secure, computer-generated records, with audit trails containing information about who performed what, and when. How to create plate definitions - Tecan The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. Celladhesion, chemotaxis, cell migration and invasion are essential topics in cancer development and tumor growth. Direct export to Excel for convenient data reduction. ***************************************************************************************. complete line of Tecan microplate readers, Windows XP Professional, Windows Vista 32 bit, Windows 7, Windows 8, Windows 10, Excel 32-Bit (2000, XP, 2003, 2007, 2010, 2013, 2016). CellTox Green Dye is an asymmetric cyaninedye that measures cytotoxicity as a result of compromised cellular membrane integrity (Figure 1). ***************************************************************************************. Tab 04 / Accessories According to the Lambert-Beer law, the amount of absorbed light is proportional to the concentration of the sample, and to the pathlength the light has to pass through when going through the sample. *************************************************************************************** Closing the plate lid effectively creates 16 quartz micro-cuvettes with a fixed path length of 0.5 mm perfect for 230, 260 and 280 nm measurements. 30190085. The Tecan GENios Microplate Reader is a bleeding edge multimode microplate reader combinng three important measurement modes - fluorescence, absorbance and glow luminescence - in a single, compact instrument. A number of video-based tutorials are provided with the Magellan package for immediate access to the softwares broad capabilities. General data import and export options for LIS and LIMS solutions as well as automated import of sample ID lists. Provides fast analysis capabilities from data reduction and curve fitting to calculation of kinetic parameters, such as the Michaelis-Menten constant. ***************************************************************************************. ***************************************************************************************. A versatile user interface and powerful data reduction capabilities enable the optimization of sophisticated experimental work as well as making routine tasks easy. Mikroplatten-Reader - Tecan Using DNA binding as an indicator of cytotoxicity mitigates this issue, providing a robust method of detection for time course experiments. Magellan Standard intended as reader control and data analysis software for research use in pharmaceutical, biotechnological and life science industries, for food analysis and veterinary applications. Spark multimode microplate reader for high performance cell - Tecan *************************************************************************************** IntroductionThe BacTiter-Glo Microbial Cell Viability Assay is a luminescence-based assay for the determination of the viability of microbial cells, using quantification of ATP as an indicator of metabolically active, viable cells (1). However, this technology suffers from a number of drawbacks, mainly due to high levels of stray light. Magellan software is ready to analyze your ELISAs and kinetic assays, delivering the following key functionalities, The Magellan software is designed for intuitive operation. Magellan Standard supports all plate formats up to 1536-wells and allows endpoint and kinetic data analysis, analysis of multi-label assays and multiple plates as well as spectra calculation and 3D scans. Dynamic interactions between proteins are key mediators of multiple cellular functions and are involved in almost all biological processes. It is useful for a broad range of applications, such as DNA or RNA quantification, nucleic acid purity assessment and labeling efficiency determination. Magellan extends to the Connect Stacker system and adds full data analysis capacity to Tecans Fluent Laboratory Automation Solution, Freedom EVO and Freedom EVOlyzer processing systems. *************************************************************************************** The NanoQuant Plate from Tecan allows researchers to measure the concentration and purity of nucleic acids of up to 16 samples simultaneously. By continuing to browse our website, you accept our cookie policy. Multimode microplate readers (MMRs) are commonly equipped with photomultiplier tubes (PMTs). i-control takes care of everything, correlating and displaying all of your results in one single data set. Generally, cell viability is assessed using vital dyes, from which viability can be concluded either directly or indirectly. SPECTRAFluor Plus Microplate Reader - Price, Specs - ArtisanTG This web page may contain reference to applications and products which are not available in all markets. In this note, data are presented from a comparison done by Affymetrix using Tecans Infinite 200 NanoQuant, Molecular Devices SpectraMax Plus and the Nanodrop ND1000 instrument. it is beneficial to miniaturize the assay, as the signal strength is not directly related to the sample. *************************************************************************************** A well-established and reliable method to determine the antioxidant capacity of a substance is the oxygen radical absorbance capacity (ORAC) assay [3]. *************************************************************************************** ***************************************************************************************. The Infinite 200 PROs luminescence filter wheel is equipped with four color filters, allowing you to perform sensitive dual-color luminescence applications such as NanoBRET, BRET2, ChromaGlo and others. Infinite M Plex, multimode microplate reader, monochromator - Tecan They provide a complete solution for periodic functional checks of Tecans portfolio of multimode readers. The AlphaScreen chemistry employs donor and acceptor beads that can be attached to various types of biologically relevant molecules. With optional temperature control, free wavelength selection and gradient filter-based wavelength scanning, it is an ideal solution for a range of applications, including ELISAs and kinetic assays. 2 Instructions For Use for SUNRISE Absorbance Reader No. This technical note describes the successful implementation of the Transcreener Fluorescence Intensity Assay on Tecans quad-4 monochromator-based Infinite M200 PRO and Tecans filter-based Infinite F200 PRO multimode reader. First aid In this step-by-step video tutorial, you will learn how to set up your Infinite 200 PRO microplate reader and install i-control software. Gain a new level of confidence in your laboratory equipment with this accurate, cost-effective and near-effortless solution. Comparing absorbance measurements between the Quad4 Monochromators-based Infinite M200 PRO and a multimode reader using photodiode array technology. Expression of the reporter protein can be accomplished by measuring the protein itself or the enzymatic activity of the protein. Sunrise customers can choose between two versions of the QC Pac according to their specific requirements. ), Fluorescence polarization (eg. Software designed for your workflow The Infinite 200 PRO plate reader family is supplied with the easy-to-use i-control software interface, allowing you quickly define a workflow for each of your applications. Spark Multimode Microplate Reader from Tecan | SelectScience This method, although often used, is labor intensive and tedious. This content is available also in other languages. This note describes the Dual Luciferase Reporter Assay performance using Tecans Infinite 200 PRO multimode reader equipped with a two channel injector. One of the most used methods for determining nucleic acid concentrations is based on measuring the optical density of a sample at 260 nm (OD260). Both excitation and emissions scans can be easily set up in the systems i-control software, allowing rapid assay optimization. General data import and export options as well as automated import of sample ID lists. *************************************************************************************** The Infinite F200 PROs filter-based absorbance module is the economical choice for standard absorbance applications, such as ELISAs and protein quantification assays (eg. How to install your Infinite 200 PRO and i-control software. Tab 01 / Overview They may range from cytotoxicity, proliferation, apoptosis and GPCR signaling assays to high-throughput screening (HTS) drug discovery applications. Among the devices suitable for measurements of small-volume samples Tecans Infinite 200 NanoQuant in combination with the NanoQuant Plate offers uncompromised performance for absorbance-based nucleic acid quantification and assessment of labeling efficiency and, in addition, can be upgraded at individual convenience with fluorescence and luminescence reading functions.The present note describes the implementation of the NanoQuant Plate for protein measurements with regard to essential assay parameters such as linearity, uniformity, and reproducibility. This video explains how to set up a simple kinetic reading in iControl software. *************************************************************************************** The luminescence optics in Tecan readers are based on a unique concept; an optical density filter in the modules filter-wheel attenuates strong signals by a factor of up to 100, but only if you really need it. Ideal for colorimetric ELISAs, kinetic assays and agglutination readings, Temperature control option for enzyme kinetics, Tunable wavelength option for greater flexibility and wavelength scanning, Powered by Magellan data analysis software, Advanced curve fitting and QC functions, plus data export to your LIS, Advanced 12-channel optics for fast results, Additional reference channel for optimizing light intensity, Prolonged lamp lifetime via auto switch-off function when not in use, Self-check of major components at instrument start-up, Three-year warranty, demonstrating Tecans commitment to quality, Designed to meet 2011/65/EU RoHS regulations, Provides FDA 21 CFR Part 11 functionality via Magellan Tracker software, A Peltier-based temperature control element to heat the plate from the bottom, An additional heating element above the plate, Free selection of 301 wavelengths in increments of 1 nm, Spectral scanning to help identify a suitable measurement wavelength, An additional reference channel for optimizing the light intensity, Auto switch-off of the light source when not in use to extend lifetime, An automated self-check of major components at instrument start-up, Qualitative and quantitative data analysis. Additionally, the efficiency of the nucleic acid labeling with dyes can be easily determined with this tool. Fluoreszenz-, Hellfeld- und digitale Phasenkontrast-Imaging in Kombination mit umfassender Kontrolle der Inkbationsbedingungen und Standard-Detektionsmodi machen das System zu einem leistungsstarken Tool fr die zellbasierte Forschung. Adherent cell types are typically analyzed through the well bottom in order to bring the cell monolayer as close as possible to the detector and avoid unspecific fluorescence noise by the overlying growth medium. Absorbance, Fluorescence, Luminescence. Practical wizards give step-by-step guidance through the software and help you to tailor your assay and calculation. Broad range of applications. Sunrise The fast, multichannel absorbance plate reader - with tunable wavelength and temperature control options - that can be tailor-made for your purposes. The absorbance module offers the flexibility to work with high or low volume samples, without compromising on your data quality. Magellan supports the complete line of Tecan microplate readers enabling quick and easy measurements of samples for both first-time and expert users alike. Inducing cell migrationIn the initial experiments, migration of cells through the FluoroBlok filter was validated by inverted microscopy; however, cell quantification is constrained by its variability, due to cell spreading in the presence of serum, and by the fact that it is very time-consuming. For additional warnings, procedures and information please refer to the Instructions for Use of your device. *************************************************************************************** The compatibility of these assay systems with Tecans Infinite M200 PRO Quad4 Monochromators-based multimode reader is demonstrated. Application packages for Spark - Tecan Infinite 200 PRO laboratory equipment pdf manual download. Supports all major curve fittings, including: QC functions, such as Levy-Jennings graphs for trending of standards and controls, Available in nine languages, including English, French, German, Italian, Spanish, Portuguese, Russian, Chinese and Japanese, Step-by-step guidance for measurement set-up, Round filters for installation into four-position filter slides, Rectangular filters for installation into six-position filter slides, Accuracy, linearity and precision of the optics (NIST traceable). In vivo kinetic studies of drug uptake across the gastro-intestinal tract (GIT) and blood-brain barrier (BBB) are valuable tools for assessing bioavailability to prospective targets. *************************************************************************************** BCA, Bradford, Lowry), DNA/RNA quantification (eg. 5.14 When unit is finished measurements, remove standard plate and place back in protective case. Magellan is an easy-to-use wizard-based data analysis software. Convenient automation adds full data analysis capacity to Tecans automated Freedom EVO and Fluent processing systems. The dynamics of the signal inhibition, expressed as the area under the curve (AUC), are used to quantify the antioxidant capacity, expressed as the ORAC value, by comparing the sample AUC to an antioxidant standard curve generated with Trolox, a water-soluble vitamin E analog. Provides audit trail, electronic signatures, electronic records and user administration. However, its application has so far been somewhat hampered by its limited dynamic range and sensitivity. Whether you choose filter- or monochromator-based optics, you can be assured that the system is designed to tackle any absorbance assay with ease. Tecan Journal - Quality control tools for IQ OQ of Tecan microplate readers, Absorbance monochromator wavelength check, Comprehensive functional checks of key measurement modes integrated on one QC plate for efficient IQOQ procedures, No sample preparation or pipetting steps necessary, Software-guided workflow helps you get started quickly, Set of basic IQOQ documents available from Tecan, Results documented in electronic form and printed reports, Summary sheet plus detailed measurement results available, Periodic recalibration of QC plates ensures consistent results. The assay is based on the competitive binding of ADP to a monoclonal antibody-terbium conjugate, and uses a homogeneous TR-FRET detection mechanism. Protein quantification is often required before proceeding with protein samples for isolation, chromatographic or electro-phoretic analysis, or immunohistochemical methods. This example is relevant to repeat measurements of the entire plate (aka pl. The optical design of the luminescence module guides light through a filter wheel equipped with both color and attenuation filters, which is then detected by a dedicated PMT. Ready To Use (Laptop not included) This fully-loaded multimode plate reader could become the future workhorse of . Read more Infinite F50 State-of-the-art eight-channel absorbance reader for accurate, reproducible and fast results. *************************************************************************************** It delivers excellent sensitivity, multiplexing capability and high format flexibility including 6 - 384-well microplates, half-area plates as well as cuvettes . We used the HP D300 Digital Dispenser, available from Tecan, for noncontact dispensing of test compound and the Tecan Infinite M200 PRO with Gas Control Module (GCM) for kinetic measurement of cytotoxicity in HepG2 cells over 72 hours. It provides functional checks of absorbance, fluorescence and luminescence reading modes, as well as for other key parameters such as temperature control and shaking where supported. *************************************************************************************** Manuals Find manuals for: Freedom EVO / Fluent . The present note describes the implementation of the NanoQuant Plate for protein measurements with regard to essential assay parameters such as linearity, uniformity, and reproducibility. *************************************************************************************** * Together with Magellan Tracker software. This device allows parallel quantification and analysis of up to 16 nucleic acid or protein samples, in sample volumes as little as 2 l. It supports a broad wavelength range from 340 to 750 nm and can measure an entire 96-well plate in less than six seconds, ensuring it is ready to meet the needs of routine diagnostic and research labs. By continuing to browse our website, you accept our cookie policy. ***************************************************************************************. : 30008746 2006-03 Document Revision No. Vitamin D testing in Mongolia, using a chromogenic ELISA and a Sunrise reader with Magellan data analysis software. This unique feature makes it easy to set up your reader for optimum performance with varying assay parameters. *************************************************************************************** These records are stored electronically and can be printed if needed. Tab 04 / Specifications How to work with the Te-Inject for the Infinite 200 PRO series. Working with a detailed manufacturer's technical drawing of the well plate when creating a plate definition avoids problems due to incorrect dimensions. This video describes how to create a simple method file in Tecan's Magellan software that will measure samples and derive concentrations for the samples using a standard curve. Optional extra can be supplied as Standard or Tracker version for all Tecan readers. By continuing to browse our website, you accept our cookie policy. *************************************************************************************** A cascade of energy transfer steps ultimately results in the generation of a strong luminescence signal at 615 nm, indicating specific binding between the molecules attached to the two bead types. Start the Tecan i-control software and connect to the instrument. To mimic these physiological conditions, it is a prerequisite for the incubation/detection device to have the capability to control atmospheric O2 levels.Bacteria were incubated at 37 C inside the measurement chamber of the Infinite 200 PRO reader, with continuous shaking, 10 % CO2 and varying O2 levels (normoxic (control), 5 % and 10 % O2). *************************************************************************************** A versatile user interface and powerful data reduction capabilities enable the optimization of sophisticated experimental work, making routine tasks easy. Samples can be easily applied using single or eight-channel pipettes, and each micro-spot can be quickly and easily cleaned to minimize the risk of cross-contamination. Two different techniques are generally used for colorimetric detection and quantification of proteins: protein-dye binding and protein-copper chelation. Beside the optimal z-position of signal and blank, the zposition for the maximum signal to blank ratio is also displayed. Its advanced design combines top and bottom heating of the microplate to minimize evaporation and achieve a good temperature distribution. This compact microplate absorbance reader is a powerful solution for DNA,RNA, and protein quantification and colorimetric ELISAs. AlphaLISA assays can be set up as sandwich or competitive immunoassays to detect and quantify molecules of interest in biological samples [1]. It offers powerful data analysis capabilities, excellent data presentation and outstanding graphical flexibility. It is based on the inhibition of oxyradical-induced oxidation of 2,2-azobis-(2- methylpropionamidine) dihydrochloride (AAPH) by substances with antioxidant properties. This allows long-term, tissue-based analyses such as cell proliferation studies offering reproducible measurements over time. Walkaway growth monitoring of Helicobacter pylori in the Infinite 200 PRO reader with Gas Control Module (GCM). This application note describes the use of the Infinite F200 PRO in combination with a commercially available ORAC assay kit, using different beverages as antioxidant samples Fluorescence-based drug sensitivity testing using 3D tumor microtissues and the Infinite M200 PRO monochromator-based multimode reader. Available for Sunrise, Infinite F50 and Infinite 200 PRO readers. Sunrise - Absorbance microplate reader - Tecan The video tutorial covers the following steps: Unboxing Hardware installation of the Infinite200 PRO Installation of i-control software This results in a dramatically limited dynamic measurement range (2). Its absorbance components are designed to offer superior performance for critical applications, such as A260/280 measurements, in a wide variety of microplates, with easy path length correction via the i-control software to improve data quality. The influence of assay parameter and instrument settings on data quality is discussed. The AlphaScreen signal is dependent on the extent of tyrosine kinase phosphorylation. Key Industries. For absorbance references on the QC plates. IntroductionCell-based assays are powerful and versatile tools for experimental design in life science research, and are frequently used for high throughput screening analysis. Optimized for glow-, flash- and multicolor-applications performed in 384-well plates. They are involved in various biological processes, functioning as important signal mediators. How to set different directories where Magellan/Magellan Pro data (mth, wsp) is saved, How to convert OVER results into numerical values in Magellan/Magellan Pro, How to use the Magellan/Magellan Pro Plate Geometry Editor, Magellan/Magellan Pro does not connect to the Sunrise reader, Warning message: Standard curve not monotone, How to update the Normfile for MultiCheck Plus after calibration. The software includes special applications for quick and easy DNA/RNA quantification and labeling efficiency determination, reducing the need for user input. Tecan Genios equipment auctions and classified ads on LabX for sale. The Infinite 200 PROs Te-Inject module comes with a 1 ml syringe, and can be equipped with one or two channels. This note describes the use of the fluorescence microplate reader Tecan Infinite F200 PRO or M200 PRO, for detection of as lias 20 pg dsDNA in a 200 l assay volume. The signal intensity is directly correlated to the degree of change in the membrane integrity. However, excess intracellular levels of ROS may result in cell and tissue damage, and are associated with degenerative diseases, most notably cancer. Implementation on Tecans Infinite 200 Multimode Reader Series. *************************************************************************************** For measurement and differentiation of small amounts of RNA and DNA, two detection systems using fluorescent nucleic acid binding dyes have been tested - the Quant-iT PicoGreen reagent to measure DNA and the RiboGreen reagent for detection of small amounts of RNA.For detection and quantification of small amounts of dsDNA and RNA within a wide range of concentrations, the fluorescence intensity measurement with the Tecan Infinite 200 series provides sensitive and accurate measurement results. ***************************************************************************************. Summary of Contents for tecan sunrise. The Infinite 200 PRO NanoQuant is the ideal solution for a broad range of applications for measuring DNA- or RNA- quantification, for quality control and labeling efficiency. The Sunrise plate reader, together with the Magellan reader control and data analysis software, is an ideal solution for a range of applications, including ELISAs, enzyme kinetic studies and many more. In this study the performance of the Infinite M200 with respect to the MTT assay, CellTiter 96 AQueous One Solution Cell Proliferation Assay and CellTiter-Blue Cell Viability Assay, respectively, was evaluated. This proven platform has now evolved into a budget-friendly reader family consisting of six application-focused configurations, delivering performance and value to every lab, every day, for every assay. Complex workflows made easy i-control allows you to create a workflow for each application, using 'drag and drop' processing steps . In addition to standard absorbance measurements, the NanoQuant Plate is compatible with top reading fluorescence measurements (eg. The plate thickness is defined by selecting the corresponding plate type in the software (see i-control Instructions for Use). The advanced design of the Sunrise plate reader includes, *************************************************************************************** A selection of application-specific absorbance filters are available, and the instruments filter slide allows you to load four absorbance filters at a time. 9 963 views 1 year ago Tecan Knowledge Portal This video tutorial provides a step-by-step guide on how to install your new Infinite 200 PRO and i-control software, from unboxing to your first. Filter-based devices provide increased sensitivity and access to high-end detection technologies, such as TR-FRET and fluorescence polarization. Infinite M Plex, multimode microplate reader, monochromator optics. We demonstrate the use of a non-activity-based cytotoxicity probe, CellTox Green Dye, which can be added at the beginning of an experiment and employed in real timefor the kinetic determination of cytotoxicity. Tecan's Sunrise absorbance microplate reader offers all the functionality needed for numerous photometric applications, including advanced 12-channel optics for fast, multichannel absorbance reading of ELISAs, a temperature control function for enzyme kinetic assays, and a tunable wavelength function for wavelength scanning. *************************************************************************************** Magellan Tracker offers all functionality for compliance with the FDA regulation 21 CFR part 11 for electronic records and signatures in addition to the functionality of Magellan Standard. Please check with your local sales representative. In this step-by-step video tutorial, you will learn how to set up your Infinite200 PRO microplate reader and install i-control software. The phthalocyanine photosensitizer molecules embedded in the AlphaScreendonor beads convert ambient oxygen into singlet oxygen when excited at 680 nm. Magellan Get more from your microplate reader with Magellan, the universal reader control and data analysis software from Tecan. Together with the Magellan Tracker software, the Sunrise plate reader is designed to meet the FDA 21 CFR Part 11 functionality. This content is available also in other languages. The tunable wavelength option delivers maximum application flexibility on one filter slide. When the dye binds to DNA, green fluorescence is enhanced. By continuing to browse our website, you accept our cookie policy. Infinite M Plex from Tecan | SelectScience It combines top and bottom heating to minimize evaporation and provides a good temperature distribution across the plate, using.
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