Use Tecan Spark multimode microplate reader to measure absorbance or fluorescence intensity of green fluorescence protein. Variability is hard to avoid, whether it is transferring your cell-based assay between instruments or manually assessing confluence. The innovative design of Sparks High Speed Monochromator delivers an absorbance spectrum between 200-1,000 nm in just five seconds using a step size of just 1 nm, effectively eliminating the risk of sample evaporation. Using traditional bottom reading for cell based fluorescence assays reduces assay sensitivity because the light needs to pass through either plastic or glass before it reaches the sample, which reduces the amount of light available for excitation. One-click applications streamline your most common assays, getting you from sample to results faster than ever before. Whether you need to quantify a nucleic acid or automate an injection at a specific absorbance value, SparkControl works for you, freeing up more time for your research. The design of taking out the injector out of the unit to prime and put it back and forth for injection is kind of difficult especially when injector is used a lot. Spark is equipped with precise temperature control to enable uniform heating of your microplate. These new options combined with the ability to read 1- to 1,536-well microplates ensure there is a Spark configuration to increase the productivity of your lab, both today and in the future. For many cell biology applications such as the identification and separation of high expression clones or knock-down candidates, and small-scale antibody or protein production it is important to ensure that cell lines originates from a single cell, i.e. Simply add ddH2O to the filling moat and you have an instant, no-fuss humidified atmosphere in your microplate chamber. The NanoQuant Plate allows parallel quantification and analysis of as little as 2 l of up to 16 nucleic acid or protein samples. The Spark was tested for its capacity to measure the Transcreener ADP2 TR-FRET Red Assay using the four different combinations of the Fusion Optics. For example, benefit from the new label-free cell counting functionality to simplify your cell-based assay workflow and save valuable time. Tecan's Spark 20M multimode microplate reader Lelystad Travel and City Guide - Netherlands Tourism photo of Munttoren (Treasury Tower) by peperbrug/Flickr. This can be a major bottleneck, especially for academic laboratories, as dedicated automated microscopy systems are both very expensive and complex to operate, meaning verification of monoclonality is often performed by manual microscopy. An advantage is its absorbance OD window is quite a lot bigger than similar readers from other manufacturers. Tecan offers microplate readers with both multimode or absorbance reading options. The influence of important instrument settings, such as shaking, are considered and discussed. Unlike hybrid multimode readers, this concept allows users to independently choose between filters and monochromators for both excitation and emission. Affordable imaging for increased data confidence. A comparison of all Tecan single- and multimode readers. Reliable results are dependent on a stable temperature. As all relevant features for environmental control are now fully integrated into the reader and the software, I am convinced the Spark will become a very valuable tool for analysis of biological processes. Achieving optimal fluorescence assays requires an optics system with both sensitivity and flexibility. This unique feature is available for all fluorescence-based detection modes including standard top and bottom measurements, TRF, FRET, TR-FRET and FP delivering very low detection limits and unlocking new possibilities. This freely configurable system gives researchers access to new techniques and features intended to enhance and streamline biochemical and cell-based workflows. Spark is convenient, fast, sensitive and easy to use. Bradford assay for protein quantification, small volume DNA or RNA quantification with NanoQuant plate, quantification of fluorescence-labeled small molecule, WST-1 assay (cytotoxicity assay, enzyme assays. Carillons in Amsterdam. The fully configurable platform also gives you the freedom to personalize your own Spark reader. Developed with cell-based assays in mind, it offers a host of software and hardware features, including a unique Te-Cool cooling module that delivers total temperature control, even below ambient. The easy to use interface and extensive capabilities make this a much used instrument. Delivering performance and value to every lab. ***************************************************************************************. SparkControl takes care of everything, correlating and displaying all of your results in one single data set. Use the Tecan Connect mobile app to monitor instrument status and alert you when user interactions are required. The Host-Microbiota Interactions Lab at the Wellcome Sanger Institute uses high throughput anaerobic microbiology, genomic and metagenomic approaches to explore the relationship between humans and their microbiome the community of microorganisms that live on and in us. The innovative design of Sparks High-speed monochromator delivers an absorbance spectrum between 200-1000 nm in just 5 sec, using a minimum step size of just 1 nm. The SPARK instrument has been the best reader for analyzing our samples, proceeded from being candidates for new drugs. The system had several issues at the beginning and needed to be replaced a couple of times. Emission spectra of Renilla (RR) and firefly (FF) luciferases recorded with the Spark. How to measure gain and z-position while doing fluorescence SparkControl offers an efficient solution: Open Kinetic. Additional features including gas and temperature control, lid handling, dual injection and, evaporation protection allows for flexible and versatile experimental design of cell-based applications. The study results can be used as a guideline to help scientists choose the right method for their specific application. Tecan | Spark | Scimed Take a last look before starting your assay. Whether you are looking to design long-term experiments that work around your schedule, attempting to shrink sample volumes to reduce costs, or trying gain access to multiple instruments on a tight budget, Spark gives you control over your priorities. Multiple consecutive scans give fluctuating results; this means, 3 consecutive scans may provide 3 random cell countings. The cell counting module achieves the step up from the microplate reader towards automated imaging. *************************************************************************************** SparkControls remote control feature allows you to monitor or operate the instrument from outside of the lab. Spark brings together a unique set of features intended to ease this strain and create new workflow possibilities for your laboratory, reducing hands-on time while producing high quality data for each and every sample. 5 March 2015. The Spark reader is equipped with Tecan's unique Fusion Optics, which combine the benefits of filters and MCRs in a single multimode reader. Spark-Stack is a compact, versatile and field-upgradeable microplate stacker for the Spark multimode reader. Latest: Environmental Analysis, Pesticides, Contaminants & Sustainability: Technologies and Latest: 6 upcoming webinars to boost your research, Latest: Materials science: Key technological advancements to celebrate. The Optimal Read function minimizes variation originating from inhomogeneous cell distribution by performing multiple measurements on spatially separated spots arrayed across each well. *************************************************************************************** With Sparks unique Te-Cool module, you can heat or cool the measurement chamber. High definition well scans give you a qualitative image of the cell distribution, effectively giving you a complete picture of the cell population. This is complemented by a patent-pending High Speed Monochromator, which allows complete absorbance scans from 200 to 1,000 nm in under five seconds; the speed of a diode array is combined with the precision and flexibility of a monochromator, offering wide-ranging and detailed spectra with unsurpassed wavelength accuracy for rapid DNA and protein quantification. One last look. SparkControl software provides effortless control of the system, enabling easy selection of filters or monochromators on the fly. Buy Tecan plate reader For Sale, New & Used Prices | Labx.com High throughput DNA quantification and quality checks for low volume samples. The injectors are easy to implement. This content is available also in other languages. Get instant access to applications and enjoy easy operation with SparkControls workflow-based interface. *************************************************************************************** Lelystad. The fully configurable platform also gives you the freedom to personalize your own Spark reader. Sparks Fusion Optics represent the first optical solution to combine the flexibility of monochromators with the sensitivity of filters in a single fluorescence measurement. SparkControl excels in handling routine or complex workflows. Tecan Sparks cell incubation features like heating, built-in gas control and humidity control allow cells to be incubated in the reader for days. TECAN support in Spain has been quietly involved in optimizing the software application to adapt it for our necessities. The resulting signal is detected by a photomultiplier tube (PMT) operated in its most sensitive mode; photon counting. *************************************************************************************** Culture medium analysis for a metabolic analysis of antibody-producing cells using LC-MS/MS and Oligonucleotide Purification and Quantification Technology. HTRF is a time-resolved fluorescence resonance energy transfer (TR-FRET)-based assay system from Perkin Elmer cisbio, and is popular for the analysis of various molecular interactions and binding studies. You can also make methods for automated enzyme assays. Whether your research is centered in academia or industry, your next discovery will rely on your data. In combination with the special read functions like lens-based bottom read, automatic z-focus and optimum read function, Tecan Spark is the ideal multimode reader when working with cells and fluorescence. The image shows OD linearity from 0 to 4 OD measured at 260 nm. But isnt your data too important to compromise? For most readers, the specified minimum temperature inside the measurement chamber is defined as a few degrees above ambient. Each aspect is meticulously manufactured to ensure that the data you produce is true and consistent, empowering your discovery. As for maintenance and product support, the representatives are always available too and are very knowledgeable and helpful. Unique heating and stirring capabilities reduce the risk of precipitation. All available optics configurations of the Sparks Fusion Optics system meet the requirements for HTRF certification and are HTRF-compatible. The machine produces good results as expected. Now the system is stable and it became a very precious tool for our research in the lab. Using the Spark is fast and easy, and the results are dependable - when it is working properly. Tab 01 / Overview A device that is easy set up, has an intuitive interface, fast and robust. It provides reliable walkaway processing of up to 50 plates per run. The detection limit for the far red fluorescent dye Alexa Fluor 647 which has a fluorescence spectrum comparable to Cy5 was determined, operating each instrument in MCR mode. Easy to use instrument for a large number of different applications. Tecan uses cookies to improve our website. Designed for users at any skill level, SparkControl uses icon-driven, drag and drop implementation. This machine fulfills what we had in mind. The plate reader is easy to use and straight forward. Bioluminescence resonance energy transfer (BRET) is a well-established detection technology for the investigation of protein-protein interactions in living cells. Keep control of crucial experimental conditions with the first air conditioning system for a multimode reader enabling stable measurement chamber temperature down to 18C. Immediate and thorough support if any problems occur. Glow luminescence linearity of the Spark using optimized conditions. Tecan provides a very high-quality after-sales care, with online service (via chat) and quick email responses. Prt pour ceux de demain. This is monitored as an additional fluorescence kinetic while simultaneously maintaining image based growth control. Spark-Reader und Software von Tecan verbessern die Durchfhrung von Fluoreszenzversuchen in Mikrotiterplatten. The instrument can also handle any model of microplates. Tecan Knowledge Portal Tecan.com Microplate Reader Liquid Handling Fluent - How To. I got a couple of quite for different machines and the Tecan seemed best value for money. We were able to automatically record metabolic processes in live cells up to 72 hrs. Tecans Spark multimode microplate reader with integrated cell imaging simplifies cell culture, featuring quality checks and signal normalization to cell confluence, thereby boosting automation of cell-based studies. The Spark cell imaging module consists of a LED brightfield illumination, a 4x objective lens, a CMOS 1.3 megapixel camera and a robust laser-based autofocus system, delivering performance to rival an automated microscope. Most plate readers use a fluorescence emission monochromator for luminescence multiplexing or scanning, leading to inferior results and an unacceptable drop in sensitivity due to attenuation. Easy selection of one of the four preset configurations for a specific application segment provides a fast solution to solve your research problems. The webinar will focus on using Promega Cell Health Assays and the Tecan Spark multimode reader to assess cytotoxicity, viability and apoptosis, including kinetic (or real-time) analyses over multiple days. The "Gain" and "RFU" parameters in Fluorescence Polarization The novel ValitaTITER assay provides a very accurate and cost-effective solution for the detection and quantification of IgG and Fc-containing proteins directly in cell culture supernatants In this application note, we show the optimal protocol for performing IgG/Fc-protein quantification using ValitaTITER assay kits with the Spark and the Infinite 200 PRO multimode plate readers. To get results from cell based assays that can be compared from experiment to experiment and well to well, you need to pay special attention to cell numbers, cell distribution and cell health. Spark multimode microplate reader for high performance cell - Tecan Simply pipette 2 l samples from your fluorescence assay for example, the Quant-iT PicoGreen dsDNA assay onto the NanoQuant Plate and use Spark to detect the fluorescence signal. Spark is equipped with a unique IR temperature sensor enabling well-by-well temperature correction during measurements, overcoming a well-known challenge of Alpha assays. Stay connected with your experiment wherever you are. This enables full system control without the need to sit in front of the reader for hours; if you are connected to the internet, then you are connected to your experiment. I would recommend the Spark to any lab looking for a versatile plate reader. The benefits of active temperature control are demonstrated on the basis of experimental data from cell-based bioluminescent and biofluorescent assays assessing cell viability, metabolism, death and signaling. Spark is the only reader that can mitigate the impact of temperature on your luminescence assays, yielding higher quality data for your valuable research. This content is available also in other languages. The user-selectable deep blocking dichroic mirrors increase sensitivity for popular dyes in the low end of the wavelength spectrum. Tab 03 / Software We have had a few issues with our plate reader that has required service multiple times in the about 2 years we have had it. This system offers an affordable solution for users wanting to enter this exciting application area. spectra, Luminescence glow, flash, multicolor, spectra, Automated live cell imaging cell counting and confluence, Liquid dispensers with reagent heater and stirrer, Evaporation protection (humidity cassette), Te-Cool (active temperature regulation 18-42 C), Tecan Connect mobile app to monitor instrument, Reduction in evaporation during your assay, Use your existing plates no need for expensive special plates, Reduced edge effects ideal for long-term kinetics, Stable conditions better cell viability, Accurate, reproducible cell counting, with flexible area selection for greater sensitivity, One-click analysis of cell number, size distribution and viability, High precision cell counting, even at low concentrations, Automated replicate processing for multiple samples in disposable Cell Chips. Der Spark-Reader kann mit einer Vielzahl an frei kombinierbaren Modulen nachgerstet werden. Most of the 9 carillons date back to the 17th century and some still give weekly concerts, usually they play classical music but there is also a chance of hearing . Upgradeable absorbance plate reader delivering sensitive results for ELISAs and low volume nucleic acid or protein quantification assays. Spark multimode microplate reader for high performance cell-based fluorescence assays, Clinical mass spectrometry sample preparation. Whether the errors are human subjectivity, environmental factors or contamination, you are influencing your results. Freedom EVO - Troubleshooting . of luminescent proteins) can be easily set - using an innovative filter-based approach, users can select any wavelength between 398 nm and 653 nm, with a step size of 15 nm and a 25 nm bandwidth. Tecans Spark multimode reader has an integrated, bright field cell imaging module. Yet, we regret that it does not go less than 18 degrees celcius. Many kinds of 96-well plates can be used but NanoQuant plates are especially useful when you measure fluorescence intensity with small volumes of 2 uL. By combining live cell imaging with industry-leading detection technologies, you now have the ability to unite qualitative and quantitative information into unique multi-parameter data sets. However, this improved biological relevance is counterbalanced by the complexity of working with a living organism. Which smart sample prep solution best fits your application? The combination of filter and monochromator in the same measurement give unprecedented flexibility without compromise on the sensitivity. This technical note outlines the use of the Spark reader in combination with the ValitaTITER assay for the quantification of IgG. The unique design of the NanoQuant Plate, with a quartz optic for each sample, negates the need for calibration and guarantees consistent performance. Spark-Pakete fr unterschiedliche Anwendungsbereiche - Tecan The pioneering design of the luminescence optics enables spectral recording of signals without compromising on sensitivity, giving you superior assay performance and preparing you for future luminescence applications. This content is available also in other languages. Tecan uses cookies to improve our website. DNA spectrum of a 50 g/ml sample after blank reduction; a 2 l sample volume was measured in Tecans NanoQuant Plate. Dies bietet Ihnen die Flexibilitt, ein System zu konfigurieren, das exakt Ihren Anforderungen entspricht. The TeCool option is quite interesting. For example, using a filter on the excitation side gives higher instrument sensitivity, significantly improving peak quality. Spark-Stack takes care of typical process steps, such as loading, unloading and re-stacking of non-lidded 6- to 1,536-well plates, and provides a throughput of up to 50 plates per run. *************************************************************************************** Unparalleled sensitivity in the widest range of applications. Combined with SparkControl software, the system extends the dynamic range with automated gain regulation to avoid fluorescence measurements running into saturation. Tecan Spark multimode microplate reader, with fluorescence Fusion Optics meets these challenges and offers everything you need to develop and run advanced biochemical and cell-based fluorescence assays. Sophisticated and high quality instrument. Schnelle Absorptionsmessung in Mikrotiterplatten und Kvetten mit Monochromator. Integrated injectors give you valuable dispensing capabilities, ideal for high sensitivity applications such as flash luminescence. We have had a few problems at the beginning but the engineers came over to have a look at it, sent it back to be fixed and provided us with a replacement demo machine in the meantime. Mehr Infos The problem is always with the same part, the cell counting module. In addition to absorbance measurements, the NanoQuant Plate can be used to detect fluorescence signals in low volume samples. The equipment is running reliably. I have been able to set up a new method to measure real time changes in extracellular pH using fluorescent probes. Spark uses luminescence fibers optimized for each plate format 96, 384 and 1,536 wells guaranteeing minimum signal crosstalk between wells. Spark Multimode Microplate Reader by Tecan.
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